Tobacco smoke-associated N7-alkylguanine in DNA of larynx tissue and leucocytes

Biologically relevant chemical reactions of N7-alkylguanine residues in DNA.

Dietary variables associated with DNA N7-methylguanine levels and O6-alkylguanine DNA-alkyltransferase activity in human colorectal mucosa.

Components of human diets may influence the incidence of colorectal adenomas, by modifying exposure or susceptibility to DNA-damaging alkylating agents. To examine this hypothesis, a food frequency questionnaire was used to assess the diet of patients recruited for a case-referent study where biopsies of normal colorectal mucosa were collected during colonoscopy and subsequently analysed for DN...

A fluorimetric assay for the spontaneous release of an N7-alkylguanine residue from duplex DNA.

A fluorimetric assay for monitoring depurination of the N7-alkylguanine adduct derived from the anticancer natural product leinamycin is described. This general approach could potentially provide the foundation for a high throughput assay that detects DNA-alkylating agents or a convenient continuous fluorimetric assay for base excision repair enzymes.

Biological activity of tobacco smoke and tobacco smoke-related chemicals.

Exposure to whole cigarette smoke from reference cigarettes results in the prompt (peak activity is 6 hrs), but fairly weak (similar to 2 fold), induction of murine pulmonary microsomal monooxygenase activity. This activity can be detected by using as substrates either benzo(a)pyrene or ethoxyresorufin, and can be inhibited by treatment with cycloheximide or actinomycin D. Unlike the induction ...

Association between tobacco smoke and serum nitric oxide metabolites concentration

Introduction: Cigarette smoking affects nitric oxide production. The aim of this study was to determine associations between tobacco smoke and serum nitric oxide metabolites (NOx) concentration. Methods: Serum NOx concentration was measured by the Griess method in 230 nonsmokers, 238 active, 196 passive and 29 waterpipe smokers. Subjects were selected from participants of Tehran Lipid and Gl...